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The effect of mutations-caused amino acid changes on protein function was predicted by a combination of tools that use sequence homology, evolutionary conservation, and protein structural information.The
- To run the functional analysis, first you need to create the variantsfile.txt
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- . For that, you will utilize the information from the Workshop_samples_05-17-23_nocont_homo_common.bim file obtained
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- in the previous step.
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- The variantsfile.txt file should
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- be structured with two columns
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- , namely Pos and Variant
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- . For further reference, please consult Figure 5.
Figure 5 - variantsfile.txt example
2. Upload the variantsfile.txt into your work directory in the SCC cluster and run the command below.
./mutserve annotate --input variantsfile.txt --annotation rCRS_annotation_2020-08-20.txt --output AnnotatedVariants.txt |
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