Overview

This is a guide for mtDNA sequencing data cleaning and analysis in Plink and R.

Learning Objectives

(1) Alignment and variants calling (from fastQ to bam files)

(2) Apply quality control measures for mtDNA genotyping data sets;

(3) Identification of homoplasmic and heteroplasmic variants;

(4) Functional mapping;

(5) Get familiarized with Plink and R environments.

## All material is located in the following folder: 

## ***Powerpoint slides for this workshop:

Please download all files and create a folder on your PC or cluster to run the analysis. Remember: plink must be stored in the same folder if running on your PC.

Resources

1. Reference files: Human genome 
   1. Download the reference files using this link https://console.cloud.google.com/storage/browser/genomics-public-data/references/hg38/v0;tab=objects?pageState=(%22StorageObjectListTable%22:(%22f%22:%22%255B%255D%22))&prefix=&forceOnObjectsSortingFiltering=false
2.  Nextflow (https://www.nextflow.io/)
   1. Need to install nextflow in your work directory by using the command: curl -s https://get.nextflow.io | bash
3. Singularity
   1. Need to load singularity module if using CAMH Specialized Computing Cluster: e.g., module load tools/Singularity/3.8.5
4. Mutserve (https://github.com/seppinho/mutserve) 
   1. Need to install mutserve in your work directory by using the command: curl -sL mutserve.vercel.app | bash
5. Our pipeline was adapted from https://github.com/gatk-workflows/gatk4-mitochondria-pipeline.
6. PLINK (http://pngu.mgh.harvard.edu/~purcell/plink/)
   1. PLINK 1.9 (https://www.cog-genomics.org/plink/)
   2. Need to load plink module if using CAMH Specialized Computing Cluster: e.g., module load bio/PLINK/1.9b_6.15-x86_64
7. R (http://cran.r-project.org/)
   1. Need to load R module if using CAMH Specialized Computing Cluster: e.g., module load lang/R/4.0.3-Python-3.8.5-Anaconda3-2020.11
8. Unix-based command terminal
9. A text editor

Before starting

• Download reference files.
• Module load Java, Singularity, and install nextflow and mutserve. 
• Clone your pipeline into your work directory: e.g. git clone pipeline_link_depository

NOTE #1: all written in red is editable.

Aligment and variants calling

• To run the alignment and variants calling use the command line below.

#NOTE: Ensure that at the end of the job were created 4 different files for each sample (.vcf, .vcf.tbi, .bam, .bai) and all_samples.csv, and if they were stored inside the folder called output.

• For each one of the samples we need to generate an unique .vcf and .txt file that are going to be used for quality control analysis. For that, use the command below.

#NOTE: It is expected to generate two files: filename.txt and filename.vcf.gz in the work directory.

#NOTE: all written in red is editable.

Alternative way to merge the vcf files

• If the previous command doesn't work you can use this alternative way to merge all the .vcf files. 

module load bio/BCFtools/1.11-GCC-10.2.0
for i in $(ls *.vcf.gz); do bcftools index --tbi $i; done
bcftools merge -Oz /work_directory/*.vcf.gz -o Merged.vcf.gz

Haplocheck

Contamination

wget https://github.com/genepi/haplocheck/releases/download/v1.3.3/haplocheck.zip
unzip haplocheck.zip
./haplocheck --out haplocheck_results Merged.vcf.gz 

Haplogrep

Haplogroup

curl -sL haplogrep.now.sh | bash
./haplogrep classify --in filename.vcf.gz --format vcf --out haplogroups.txt

• Before go to the Quality Control steps, please download the all_samples.csv and filename.txt files to your computer.

QUALITY CONTROL 

• Open the all_samples.csv and filename.txt  files in excel.